Introduction Much of the background information for this set of experiments can be found in my previous post. In short, endothelial cells mainly express two receptors to the sphingolipid S1P, S1PR1 and S1PR3. S1PR1 is characterized as a barrier enhancing signaler, while S1PR3 appears to elevate vascular permeability while promoting leukocyte capture. Based on TEER…

Thanks to Dan Ahmad for performing the filtrations. Introduction I want to see if we can improve the sample preparation method for the recovery of SARS-COV-2 from wastewater filtration using our nanomembrane technology. Currently, raw samples are ultracentrifuged against a sucrose cushion to create a pellet that can be used with a RNA extraction kit,…

This post is a continuation of the project found here:  Fabricating 500 nm Channels in Silicon Wafers. The chief limitation of the previous device design was that the long narrow nanochannels were not effectively wetted. To combat this problem, we would like to fabricate the channels in situ on top of NPN. This could be…

Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test both factors concurrently we designed experiments in an attempt to capture 1.24 um diameter fluorescent…

 Goal: Is to produce a membrane has pockets to capture the extracellular vesicles EV’s. Membrane structure: Figure 1 shows the membrane structure. The membrane will have two pores sides: upper and lower. The pores on top are bigger than the bottom and the diameter of the pores should be similar to or larger than the…

Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which are collected in the last step of the isolation process. I began by seeding human…

Goal In preparation for experimentation with hCMEC/D3, an immortalized BBB cells line, I characterized barrier formation by taking TEER. I want to find peak barrier formation to determine what day to run assays. Methods hCMEC/D3 were maintained and passaged in EGM-2 (EBM-2 + multiple gfs + gentamicin + 2.5% FBS) with growth factor concentrations matching…

In the study, which combines the compressed sensing (CS) and the structured illumination microscopy (SIM) methods, an object plane should first be collected by a lens, and then the collected signal should be measured by a photomultiplier tube (PMT), located at the focal point of the lens. The recorded measurements present a measurement vector that…

Hi all,   I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting

Summary There’s a lot to be desired in counting pores in grayscale images, especially when the contrast differences are low. A human eye is usually much better at picking out slight differences in contrast, and this can mean the difference between an open and closed pore. Differences of 5 nm can be significant in certain…