To follow up on my recent posts about HUVEC YAP localization after 24 and 72 hours (https://trace-bmps.org/blog/data/2016/10/19/yaptaz-passage-one-staining and https://trace-bmps.org/blog/data/2016/10/11/yaptaz-staining-on-membranes-and-nonporous-sio2) , we conducted the same study using Adipose Derived Stem Cells. Due to the fact that these are mesenchymal stem cells, we thought that their expression of YAP might be a bit different due to the…
(This post is not finished, but my flight keeps getting delayed in 20 minute increments, so better to publish it unfinished than to not get it up before NRG in case I’m not able to make it. Please forgive typos etc.) I attended a meeting organized by the Kidney Health Initiative, a collaboration between the…
This is pretty much an update to a previous post on trying to identify exosomes using an immunogold staining procedure, FIB lift-out and the TEM. After multiple attempts to cut out a section of the membrane for attachment to a TEM grid (we were able to cut it out and weld it to a grid…
Hello Everyone, Following my experiments with Track Etched Membranes: So we came up with this idea to perform SEM imaging. Following are the images that i took for my experiment with 100 nm beads over 100 nm pores after releasing step: So in this image, we can see that there are a lot of beads…
There are two longitudinal studies for Chronic Kidney Disease (CKD). Ancillary studies can be set up to share the collected data, bio samples, or even request additional data to be collected. These Ancillary studies can be funded through normal channels, NIDDK, ASN, VA, etc. Approval to use the data should be requested 3-4 months before…
Purpose: Study the hemocompatibility of different substrates using platelet activation as the indicator. Extraction of platelet rich plasma (PRP): PRP were prepared from whole blood following the standard protocol outlined by Abcam (http://www.abcam.com/protocols/isolation-of-human-platelets-from-whole-blood). To describe briefly, 9 mL whole blood was centrifuged at 200g for 20 minutes in a 15 mL Falcon tube to obtain…
Hello Everyone, I have been working on 100 and 50 nm beads since ACNMM conference to optimize our isolation technique for exosomes and verify that our approach works. Technically, based on the discussion that we had before about Peclet Number, in order to decrease this number, I have been running all my experiments with 1…
While the latest small animal studies was meant to be a test of the new 1-chip bonded HD devices, device failure resulted in the use of the older 2-chip 10-channel HD devices as used on previous experiments. Here is all the data, Sham, commercial, 75 nm NPN trial 1 and trial 2. Trial 1 had…
Hopefully in the next week we will be submitting the following manuscript … X. Li, D. Johnson, W. Ma, H. Chung, J. Getpreecharsawas, J.L. McGrath, A.A. Shestopalov, Modification of Nanoporous Silicon Nitride with Stable and Functional Organic Monolayers, ACS Chemistry of Materials (2016) submitted. Here are the highlights … Traditional silanization of silicon materials uses…
I’ve been gathering data for a paper using my microporous MgF2 nanomembranes so here are some sample figures for such a paper. Herein, we are trying to create a porous, Raman-compatible substrate for use in cell culture. Researchers have used Raman microspectroscopy to perform quantitative non-invasive measurements of biomolecules in cells. It’s a very exciting…