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Nanomembrane Research Group
  • NRG

    HUVEC migration and Fibronectin Fibrillogenesis

    ByHenry January 31, 2017January 31, 2017

    Intro: Hi all, as we investigate the formation of HUVEC focal adhesions on porous SiO2 membrane , we also looked at fibronectin fibriollogenesis. Interestingly, it appears that after 24 hr (since the initial seeding), the fibronectin fibrils formed by HUVEC vary depending on the pore characteristics of the SiO2 membrane. In this case, we looked at…

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  • NRG

    (Almost) Final Versions of Model Paper Figures

    ByKarl Smith January 31, 2017January 31, 2017

    We’re submitting the following paper to Separations and Purifications within the next two weeks: model-paper model supplement Below are the (almost) final versions of the figures from the paper. Figure 1: Schematic of Particle Distribution for Particles Rejected by Membranes The distribution is a quasi-equilibrium between convection towards the membrane and back diffusion, which is…

    Read More (Almost) Final Versions of Model Paper FiguresContinue

  • NRG

    Partial Etch RIE Blows out NPN Pores

    ByGreg Madejski January 31, 2017September 19, 2017

    As the title states, this post is about shifting an arbitrary pore distribution in an NPN layer. Why would we want to do this? We believe that the underlying pore distribution and shape influences the properties of many of our devices (my SERS sensor work included). Jim has coined the term ‘bowl-with-a-hole’ to describe the…

    Read More Partial Etch RIE Blows out NPN PoresContinue

  • NRG

    Final Update over TE membrane

    ByAslan January 30, 2017January 30, 2017

    Hello Everyone,   So first of all, a quick reminder of what i have been working on: we were able to show the trapping of the beads by TE membranes, although in final step and after releasing of the beads, we could still see beads on the membrane, so we hypothesized that the beads are…

    Read More Final Update over TE membraneContinue

  • NRG

    Quick Characterization of 3D Printed Reverse Sepcons

    ByKarl Smith January 24, 2017January 30, 2017

    Photo Courtesy J. Adam Fenster, U of R We’ve had several of each of the two prototype reverse Sepcon designs professionally 3D printed for us (top photo). I did a few quick tests to see how they worked in the lab. “Fixed” Version This is designed to snap onto the outer lip of a 2…

    Read More Quick Characterization of 3D Printed Reverse SepconsContinue

  • Knowledge

    A Summary of Blender Tutorials for Creating Cover Art

    ByGreg Madejski January 19, 2017January 19, 2017

    Blender is a free, open-source software package for modeling 3d objects. While Sketchup is much easier to learn, it has a number of limitations that make it more difficult to do more detailed modeling. Blender has a plethora of features, including cloth, particle, and water simulation, that make it very useful. You can also design and…

    Read More A Summary of Blender Tutorials for Creating Cover ArtContinue

  • NRG

    Rotation Project: The Influence of Collagen Gels and Nanoporous Chips on Shear Based Endothelial Cell Alignment

    ByAlec January 17, 2017February 11, 2019

    As an introduction, my name is Alec Salminen. I received my BS in Biomedical Engineering at the University of Arkansas and am currently in my first year of the BME PhD program at the University of Rochester. As my rotation in the McGrath lab gets underway, I would like to share with the group my…

    Read More Rotation Project: The Influence of Collagen Gels and Nanoporous Chips on Shear Based Endothelial Cell AlignmentContinue

  • NRG | Protocols

    Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane

    ByDan Steiner January 16, 2017February 11, 2019

    Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that will provide more effective human dialysis treatment (Johnson et al. 2013). For initial tests, a…

    Read More Rotation Project Complete: Dialysis of cytochrome c across a commercial membraneContinue

  • NRG

    MANUSCRIPT REVISION: Membrane pore spacing can modulate endothelial cell-substrate and cell-cell interactions

    ByThomas Gaborski January 16, 2017January 31, 2017

    Membrane pore spacing can modulate endothelial cell- substrate and cell-cell interactions (REVISION) We submitted our “focal adhesions” paper to ACS Biomaterials Science and Engineering late last year. We received reasonable reviewer requests from three reviewers. I think I am mostly finished with revising the figures. Here are the updated figures and my comments on what changed….

    Read More MANUSCRIPT REVISION: Membrane pore spacing can modulate endothelial cell-substrate and cell-cell interactionsContinue

  • NRG

    NanoAuger Analysis of Magnesium Fluoride Nanomembranes: The Hunt for Silicon Nitride

    ByKilean January 16, 2017

    This is my third post that is related to the NanoAuger system that I had the opportunity to use while I was home over break. However, instead of quantum dots and exosomes, this time I will be talking about data pertaining to Greg’s magnesium fluoride membranes that are used for Raman imaging. And since I…

    Read More NanoAuger Analysis of Magnesium Fluoride Nanomembranes: The Hunt for Silicon NitrideContinue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore