Makerware 3d print Preview
One neat feature of the Makerware software is to visualize the printed layers before actually printing them. You can catch a lot of errors with this feature; I highly recommend it.
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Importing Gasket Geometries to Silhouette from Sketchup
We currently use Adobe Illustrator to design silicone gasket geometries for our microfluidic devices. Here is a quick way to do a similar thing from sketchup. The advantage of this procedure is that you can create a complete 3d model of your device and export different snapshots to the 3d printer for bulk fabrication and a…
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TEOS film stress
Kelly & I deposited 100 & 300 nm thick SiO2 films on bare wafers with the P5000 PE-CVD tool at RIT last week. The wafers were then annealed according to JP’s recipe overnight to stabilize the TEOS layer film stress. The stress was measured using the Tencor P2 profilometer by scanning across it before depositing…
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Using Standard Deviation to Determine Sig Figs
As part of the decision letter on Barrett’s ancient vacuole manuscript, the editor sent a little tutorial from the University of Texas on significant digits and standard deviations. Apparently ACTA Biomaterialia looks at this carefully and found we needed a little schooling. If I’ve seen this approach to the topic of significant figures, I’ve long…
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Cell growth in micovasculature mimetic device
I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN chip in sepcon format so that I don’t have to be restricted by number of…
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Creating Endothelial Cells from Adipose-Derived Stem Cells
In conducting experiments to address reviewer comments, we again created endothelial-like cells from adipose derived stem cells (ADSCs). As in the past, the vast majority of ADSCs express some CD31 (PECAM-1) after culture in endothelial media (M200) supplemented with 50ng/mL VEGF (on 0.5LP, 3.0LP and TCP). The expression of this CD31 is relatively low and…
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Sham Dialysis Study
We performed four hours of sham dialysis, using two non-porous nitride membranes. The set-up is described in an earlier post. Blood samples were taken when the animal was received, after one week of the adenine containing diet, then at 1 hour intervals during the dialysis. The data shows that the urea level, which was increased over…
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HUVEC spreading on various microporous SiO2 membranes
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future post we will quantify the degree of spreading at 1hr, 4hrs and 24 hrs. Calculation…
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IV curve analysis of 20 nm NPN
These chips were 2.9 mm TEM grid with a single 25 um window. 20 nm thick NPN is covered with 100 nm TEOS with a 2 um opening to expose ~1000 pores. Wafer number 2176. Following are IV curve analyses for the above material as well as membranes treated with ALD alumina. These were measured…
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The New Concentration Polarization Model Turns Out To Be Difficult To Implement In Matlab
This is part three of a series of posts on constructing a mathematical model of the movement of gold through a porous membrane. In part one, I implemented William Deen’s mathematical model of the electrostatic and steric effects that contribute to whether a gold nanoparticle will translocate through a pore. The mathematica and matlab code…