I went over to RIT to work with JP on measuring the deflection of NPN and pnc-Si membranes as a function of pressure. JP and Dave Fang did this work years ago, and we want to pick it up again to correlate with Sarah’s burst pressure data. We used the Veeco Wyko (white light interferometer)…
This past year I have been collecting burst pressure data on pnc-Si and NPN. The data for 30nm pnc-Si came from my previous post, “Wafer 1059: Burst Pressure Analysis”, which also includes a wafer map. This wafer had 7% porosity and an average pore diameter of 20nm. I tested square window sizes with side lengths of 0.1mm,…
With the help of Henry and Greg, the Fisher team has been busy learning the basics. John and Ryan have learned some PDMS microfluidics-making and will have their first chips ready on Tuesday. We have also been playing with the silhouette printer, studying our options for producing some simple devices. Finally, we produced some chip designs to…
I’ve been going to considerable lengths to try and get reproducible zetapotential measurements from my streaming potential device. Zetapotential measurements are the closest we can experimentally get to measuring the surface charge of a membrane, and are important for three reasons. First, I need accurate surface charge data for the membranes if my electrostatic model is…
We are carrying out filtrations using wafer 1085. We are testing the total volume of filtrate that passes in a given time. We expect the total filtrate volume to decrease as concentration increases due to more immediate clogging. Ultimately, we want to show that we can get higher total volumes of filtrate in the reverse…
I am trying to create ITO electrodes for my microfluidic device for TEER measurement system. In my previous posts, I have shown that silver/silver chloride electrode, which is most commonly used in most commercial TEER systems, had killed the cells when grown with the electrode. So Greg and I were trying to optimize the recipe…
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This week I’ve added male connectors to my device. Originally, I had female-female tubing connections to the device, which were very prone to leaking around the insertion ports. Tejas suggested that I try printing out the male connectors to combat this problem. The tubing I’m using is ID=2 mm, OD= 5mm. It’s compliant enough to…
The overarching goal of this collaboration is to expose students and faculty at primarily-undegraduate institutions to the technology & expertise available at the University of Rochester. We would like to make this kind of experience a recurring theme at URNano, and we expect to lay the foundations of such a program during the Summer of…
The goal of the experiment was to use a fluorescent dye to label live cells in order to measure proliferation over several days. To avoid cell toxicity, the intensity of the fluorescence was reduced to ~1.5%. Human umbilical vein endothelial cells (HUVECs) were seeded in a a 96-well plate at a density of 5000 cells…