Just before BMES I was finally able to electrically characterize my NFT chips. Previously, I had been trying to collected IV sweeps using the following circuit: The problem with this was that, as Jess discovered, the membranes have almost no electrical resistance in this configuration, and I was essentially shorting out the circuit. This meant…
In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are attracted to something in the Geltrex causing them to migrate. The ADSC did not migrate…
Here are my thoughts on the talks I attended at BMES: OP-Thurs-1-11. BioMEMS I Oral Thursday, October 23, 2014 8:00 AM – 9:30 AM Room 008A Moderator 1: James Tunnell Moderator 2: Catherine Klapperich 8:15 AM – 8:45 AM High-throughput High-content Developmental Biology and Neurogenetics H. Lu. Abstract This talk used a number of image processing…
After a lot of trial and error with the hinge, I was able to create something usable!!! Apparently I cannot upload the .stl file or .skp file to the blog directly for “Security Reasons” I printed this one out of Makerbot ABS at 228 C (build plate 115 C), with 0.15 mm layer height slicing,…
Our motivation was to look into how ADSC influence HUVEC network formation. My first objective was to investigate the optimal cell count, the best volume of Geltrex and if it was better to use M200 media with or without growth supplements. I then looked into seeding HUVEC on a nonporous membrane. At the same time,…
Recently, Jim has purchased a Makerbot 2x printer for our group. I’ve spent the last few weeks printing and debugging. It has the capability to print many different types of material, along with 2 separate nozzles. I have been able to get many fine prints done with ABS plastic, and I’ve put together a basic…
We’ve been moving to a new format for chips, trying to standardize an active area that has good yield and allows fluidic access through the chip itself. The SEPCON outer dimensions have been reused (5.4 mm x 5.4 mm). It’s a 1.5 mm^2 single slot chip area (2 mm x 0.7 mm) with vias that…
A bit of background: In order to quantify how permeable a tissue barrier is, we use a method known as trans epithelial electrical resistance (TEER). Cells are grown on a permeable growth substrate in a transwell format. When the cells are not confluent, there is a continuous diffusion of ions across the two sides of…
I believe that we can use MgF2 nanomembranes as electroosmotic pumps, along the same lines as pnc-Si and NPN materials. To first get a sense of how much pumping I would expect, I wanted to know what the zeta potential of these membranes would be, as the potential is directly proportional to the amount of…
In our last post we showed that this latest wafer with 36 nm pores did a better job of blocking 20 nm NPs in the forward and reverse orientations, whereas an older wafer (1070- 45 nm, 15%) showed better separations in reverse orientation. Here is a couple plots summarizing the difference: So 20 nm NPs…