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Membrane Edges with SEM
I was motivated to scan our membrane edges after looking at this image from the paper Maryna presented last week: I found a couple of pnc-Si samples with membranes still attached to the edges. I also found one sample with a membrane split almost down the middle. I coated the samples with 3nm of Au…
NSF/GOALI- URNano-Fisher projects/goals
ByFernandoThe overarching goal of this collaboration is to expose students and faculty at primarily-undegraduate institutions to the technology & expertise available at the University of Rochester. We would like to make this kind of experience a recurring theme at URNano, and we expect to lay the foundations of such a program during the Summer of…
Absorbance Problems with Colored Centrifuge Tubes
This is a follow up to Josh’s post, “Continued Au Separations with SiN-NP wafer #’s 1029 and 1030”, and seems to explain why we initially got an S value greater than one during our gold separations. We conducted tests to see if the colored centrifuge tubes were at fault for our seemingly large filtrate absorbance…
Notes from PSST 2014
The International Conference for Porous Semiconductors Science and Technology, 2014, was last week in Benidorm, Spain. Jim was kind enough send me to represent NRG. Dave Fang gave a great talk at PSST based on pnc-Si data in 2010. Here is a pdf of all the abstracts: PSST 2014-Abstracts A quick summary would break the…
Effect of Membrane Morphology on System Capacity During Normal Flow Microfiltration (review)
pdf of Zydney Paper This paper tackles the concept of membrane capacity – the amount of fluid a membrane can pass before it’s flux declines (due to pore blockage or the buildup of a cake) to some arbitrary fraction of the initial flux. Common practice in industry (according to the paper) is to calculate capacity…
Closed System Device
One aspect of the blood brain barrier device is that it be a closed flow system. This would not only better mimic an in vivo environment, but also maintain Sema4D activity, negatively affecting endothelial cell junctions and providing a target for the antibody. The microporous membranes were not yet available, so a closed flow system…
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It’s not just that dimers can survive the SDS, it’s that there’s “an equilibrium between monomeric and dimeric cyt c in the presence of SDS” – this is shown in figure S1 curve F and G. Adding SDS to purely monomeric solutions makes dimers, adding it to purely dimeric solutions makes monomers. This means we can’t clean up the figures by simply starting with a more pure solution of monomeric cyt c.