Josh Miller came up with the idea of filling individual damaged membrane channels with PDMS so the rest of the chip could be salvaged. We worked on it this week, mostly Josh, and we developed a process using strips of silicone laid on the top (flat) side of the damaged channel and PDMS injected onto…
I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…
Introduction In an effort to move forward with a ‘lung microvasculature on a chip’ model, I have been experimenting with using primary human pulmonary microvascular endothelial cells in our devices. The cells (HPMECs) are purchased from Promocell and are collected from a single donor lung. Unfortunately I found little information on their use in recent…
Jirachai’s recent post on the formation of Au nanoparticles on the surface of the NPN is very exciting. For one, it is a very dense monolayer of particles. This self assembly mechanism is magnificent whereas if we tried to dry down a layer of particles, we would get a cake layer similar to what Josh…
It has been a constant theme in our EO efforts as well as our energy harvesting efforts that our electrodes need to be as close as possible to the surface to see an effect. In Jess’s early EO work, we found that our chips could be used as EO pumps that were vastly more thermodynamically…
Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas are rich in information and need a significant amount of focus to tackle, we do…
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It’s not just that dimers can survive the SDS, it’s that there’s “an equilibrium between monomeric and dimeric cyt c in the presence of SDS” – this is shown in figure S1 curve F and G. Adding SDS to purely monomeric solutions makes dimers, adding it to purely dimeric solutions makes monomers. This means we can’t clean up the figures by simply starting with a more pure solution of monomeric cyt c.